| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 37.5pg/mL |
| Detection Limit: | 62.5~4000pg/mL |
| Short Description : | This human HMOX1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | HMOX1 |
| Gene ID: | 3162 |
| Uniprot ID: | HMOX1_HUMAN |
| Specificity: | This kit recognizes Human HO1 in samples. No significant cross-reactivity or interference between Human HO1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). |
| Post Translational Modifications | A soluble form arises by proteolytic removal of the membrane anchor. |
| Function | Heme oxygenase 1: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis. Heme oxygenase 1: (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy. Heme oxygenase 1 soluble form: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. |
| Protein Name | Heme Oxygenase 1 Ho-1 Cleaved Into - Heme Oxygenase 1 Soluble Form |
| Database Links | Reactome: R-HSA-189483 Reactome: R-HSA-6785807 Reactome: R-HSA-844456 Reactome: R-HSA-917937 Reactome: R-HSA-9609523 Reactome: R-HSA-9660826 Reactome: R-HSA-9707564 Reactome: R-HSA-9707587 Reactome: R-HSA-9707616 Reactome: R-HSA-9818027 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Type Iv Membrane Protein Cytoplasmic Side |
| Alternative CLIA Names | Heme Oxygenase 1 CLIA kit Ho-1 Cleaved Into - Heme Oxygenase 1 Soluble Form CLIA kit HMOX1 CLIA kit HO CLIA kit HO1 CLIA kit |
| Specificity | This kit recognizes Human HO1 in samples. No significant cross-reactivity or interference between Human HO1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 97-111 | 103 |
| EDTA plasma(n=8) | 93-106 | 100 |
| Cell culture media(n=8) | 84-98 | 91 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 220.38 | 577.95 | 1706.82 | 231.85 | 593.77 | 1749.08 |
| Standard deviation | 19.64 | 49.99 | 176.31 | 20.01 | 52.43 | 136.78 |
| CV (%) | 8.91 | 8.65 | 10.33 | 8.63 | 8.83 | 7.82 |