• Human HLA-B27 (Leukocyte Antigen B27) Sandwich ELISA (STJE0002021)

Human HLA-B27 (Leukocyte Antigen B27) Sandwich ELISA (STJE0002021)

SKU:
STJE0002021

£194.50 - £368.50
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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This HLA-B27 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: HLA-B
Gene ID: 3106
Uniprot ID: HLAB_HUMAN
Specificity: This kit recognizes Human HLA-B27 in samples. No significant cross-reactivity or interference between Human HLA-B27 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Hla Class I Histocompatibility Antigen - B Alpha Chain
Human Leukocyte Antigen B
Hla-B
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-1236977
Reactome: R-HSA-198933
Reactome: R-HSA-2172127
Reactome: R-HSA-6798695
Reactome: R-HSA-877300
Reactome: R-HSA-909733
Reactome: R-HSA-9705671
Reactome: R-HSA-983170
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Endoplasmic Reticulum Membrane
Alternative ELISA Names Hla Class I Histocompatibility Antigen - B Alpha Chain ELISA kit
Human Leukocyte Antigen B ELISA kit
Hla-B ELISA kit
HLA-B ELISA kit
HLAB ELISA kit
Specificity This kit recognizes Human HLA-B27 in samples. No significant cross-reactivity or interference between Human HLA-B27 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-102 97
EDTA plasma (n=8) 87-102 93
Cell culture media (n=8) 90-103 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.99 1.86 8.56 0.97 1.91 8.52
Standard deviation 0.06 0.09 0.44 0.05 0.10 0.36
CV (%) 6.06 4.84 5.14 5.15 5.24 4.23
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance