| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
14.13pg/mL |
| Detection Limit: |
39.06~2500pg/mL |
| Short Description: |
This HE4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
WFDC2 |
| Gene ID: |
10406 |
| Uniprot ID: |
WFDC2_HUMAN |
| Specificity: |
This kit recognizes Human HE4 in samples. No significant cross-reactivity or interference between Human HE4 and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. |
| Function | Broad range protease inhibitor. |
| Protein Name | Wap Four-Disulfide Core Domain Protein 2Epididymal Secretory Protein E4Major Epididymis-Specific Protein E4Putative Protease Inhibitor Wap5 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Wap Four-Disulfide Core Domain Protein 2 ELISA kitEpididymal Secretory Protein E4 ELISA kitMajor Epididymis-Specific Protein E4 ELISA kitPutative Protease Inhibitor Wap5 ELISA kitWFDC2 ELISA kitHE4 ELISA kitWAP5 ELISA kit |
| Specificity | This kit recognizes Human HE4 in samples. No significant cross-reactivity or interference between Human HE4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-99 | 92 |
| EDTA plasma (n=8) | 88-100 | 94 |
| Cell culture media (n=8) | 88-102 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 126.50 | 380.77 | 1247.35 | 128.94 | 390.90 | 1196.14 |
| Standard deviation | 8.41 | 17.52 | 67.23 | 7.56 | 17.12 | 64.11 |
| CV (%) | 6.65 | 4.60 | 5.39 | 5.86 | 4.38 | 5.36 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
