• Human HDAC4 (Histone Deacetylase 4) Sandwich ELISA Kit Ready-To-Use (STJE0015799)

Human HDAC4 (Histone Deacetylase 4) Sandwich ELISA Kit Ready-To-Use (STJE0015799)

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STJE0015799

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.55ng/mL
Detection Limit: 1.56-100ng/mL
Short Description: This HDAC4 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: HDAC4
Gene ID: 9759
Uniprot ID: HDAC4_HUMAN
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues by CaMK2D is required for the interaction with 14-3-3. Phosphorylation at Ser-350, within the PxLPxI/L motif, impairs the binding of ANKRA2 but generates a high-affinity docking site for 14-3-3. Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1.
Protein Name Histone Deacetylase 4
Hd4
Database Links Reactome: R-HSA-2122947
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-350054
Reactome: R-HSA-4090294
Reactome: R-HSA-4551638
Reactome: R-HSA-8941284
Reactome: R-HSA-8951936
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between The Nucleus And The Cytoplasm
Upon Muscle Cells Differentiation
It Accumulates In The Nuclei Of Myotubes
Suggesting A Positive Role Of Nuclear Hdac4 In Muscle Differentiation
The Export To Cytoplasm Depends On The Interaction With A 14-3-3 Chaperone Protein And Is Due To Its Phosphorylation At Ser-246
Ser-467 And Ser-632 By Camk4 And Sik1
The Nuclear Localization Probably Depends On Sumoylation
Interaction With Sik3 Leads To Hdac4 Retention In The Cytoplasm
Alternative ELISA Names Histone Deacetylase 4 ELISA kit
Hd4 ELISA kit
HDAC4 ELISA kit
KIAA0288 ELISA kit
output

Information sourced from Uniprot.org

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