| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.065ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This HDAC2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
HDAC2 |
| Gene ID: |
3066 |
| Uniprot ID: |
HDAC2_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Widely expressed.lower levels in brain and lung. |
| Post Translational Modifications | S-nitrosylated by GAPDH. In neurons, S-nitrosylation at Cys-262 and Cys-274 does not affect enzyme activity, but induces HDAC2 release from chromatin. This in turn increases acetylation of histones surrounding neurotrophin-dependent gene promoters and promotes their transcription. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching. |
| Function | Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Also deacetylates non-histone targets: deacetylates TSHZ3, thereby regulating its transcriptional repressor activity. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A. In addition to protein deacetylase activity, also acts as protein-lysine deacylase by recognizing other acyl groups: catalyzes removal of (2E)-butenoyl (crotonyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation and de-2-hydroxyisobutyrylation, respectively. |
| Protein Name | Histone Deacetylase 2Hd2Protein Deacylase Hdac2 |
| Database Links | Reactome: R-HSA-193670Reactome: R-HSA-2122947Reactome: R-HSA-2644606Reactome: R-HSA-2894862Reactome: R-HSA-3214815Reactome: R-HSA-350054Reactome: R-HSA-427389Reactome: R-HSA-427413Reactome: R-HSA-4551638Reactome: R-HSA-6804758Reactome: R-HSA-73762Reactome: R-HSA-8943724Reactome: R-HSA-9022692Reactome: R-HSA-9022699Reactome: R-HSA-9615017Reactome: R-HSA-9619665Reactome: R-HSA-9679191Reactome: R-HSA-9701898Reactome: R-HSA-983231 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative ELISA Names | Histone Deacetylase 2 ELISA kitHd2 ELISA kitProtein Deacylase Hdac2 ELISA kitHDAC2 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
