| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This HCRT Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
2.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
HCRT |
| Gene ID: |
3060 |
| Uniprot ID: |
OREX_HUMAN |
| Specificity: |
This kit recognizes Human OXA in samples. No significant cross-reactivity or interference between Human OXA and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Specific enzymatic cleavages at paired basic residues yield the different active peptides. |
| Function | Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A: Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner. Orexin-B: Binds to orexin receptor HCRTR2/OX2R only. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner. |
| Protein Name | Hypocretin Neuropeptide PrecursorHypocretinHcrtOrexin PrecursorPrepro-OrexinPreprohypocretin Cleaved Into - Orexin-AHypocretin-1Hcrt1 - Orexin-BHypocretin-2Hcrt2 |
| Database Links | Reactome: R-HSA-389397Reactome: R-HSA-416476 |
| Cellular Localisation | Rough Endoplasmic ReticulumCytoplasmic VesicleSynapseAssociated With Perikaryal Rough Endoplasmic Reticulum As Well As Cytoplasmic Large Granular Vesicles At Synapses |
| Alternative ELISA Names | Hypocretin Neuropeptide Precursor ELISA kitHypocretin ELISA kitHcrt ELISA kitOrexin Precursor ELISA kitPrepro-Orexin ELISA kitPreprohypocretin Cleaved Into - Orexin-A ELISA kitHypocretin-1 ELISA kitHcrt1 - Orexin-B ELISA kitHypocretin-2 ELISA kitHcrt2 ELISA kitHCRT ELISA kitOX ELISA kitPPORX ELISA kitPPOX ELISA kit |
| Specificity | This kit recognizes OXA in samples. No significant cross-reactivity or interference between OXA and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-106 | 97 |
| EDTA plasma(n=8) | 93-111 | 101 |
| Cell culture media(n=8) | 88-100 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 188.50 | 568.10 | 1493.00 | 170.80 | 598.40 | 1389.60 |
| Standard deviation | 10.20 | 25.60 | 62.70 | 8.50 | 31.70 | 48.60 |
| CV (%) | 5.41 | 4.51 | 4.20 | 4.98 | 5.30 | 3.50 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
