| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 7.85 pg/mL |
| Detection Limit: | 15.63-1000 pg/mL |
| Short Description: | This HBEGF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | HBEGF |
| Gene ID: | 1839 |
| Uniprot ID: | HBEGF_HUMAN |
| Specificity: | This kit recognizes Human HBEGF in samples. No significant cross-reactivity or interference between Human HBEGF and analogues was observed. |
| Sample Type: | serum, plasma and other biological fluids |
| Post Translational Modifications | Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active. O-glycosylated with core 1 or possibly core 8 glycans. Thr-47 is a minor glycosylation site compared to Thr-44. |
| Function | Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. |
| Protein Name | Proheparin-Binding Egf-Like Growth Factor Cleaved Into - Heparin-Binding Egf-Like Growth FactorHb-EgfHbegfDiphtheria Toxin ReceptorDt-R |
| Database Links | Reactome: R-HSA-1227986Reactome: R-HSA-1236394Reactome: R-HSA-1250196Reactome: R-HSA-1250342Reactome: R-HSA-1250347Reactome: R-HSA-1251985Reactome: R-HSA-1257604Reactome: R-HSA-177929Reactome: R-HSA-179812Reactome: R-HSA-180292Reactome: R-HSA-180336Reactome: R-HSA-182971Reactome: R-HSA-1963640Reactome: R-HSA-1963642Reactome: R-HSA-212718Reactome: R-HSA-2179392Reactome: R-HSA-2219530Reactome: R-HSA-5336415Reactome: R-HSA-5638303Reactome: R-HSA-5673001Reactome: R-HSA-6785631Reactome: R-HSA-6811558Reactome: R-HSA-8847993Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8857538Reactome: R-HSA-8863795Reactome: R-HSA-9009391Reactome: R-HSA-9634638Reactome: R-HSA-9664565Reactome: R-HSA-9665686 |
| Cellular Localisation | Heparin-Binding Egf-Like Growth Factor: SecretedExtracellular SpaceMature Hb-Egf Is Released Into The Extracellular Space And Probably Binds To A ReceptorProheparin-Binding Egf-Like Growth Factor: Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative ELISA Names | Proheparin-Binding Egf-Like Growth Factor Cleaved Into - Heparin-Binding Egf-Like Growth Factor ELISA kitHb-Egf ELISA kitHbegf ELISA kitDiphtheria Toxin Receptor ELISA kitDt-R ELISA kitHBEGF ELISA kitDTR ELISA kitDTS ELISA kitHEGFL ELISA kit |
| Specificity | This kit recognizes Human HBEGF in samples. No significant cross-reactivity or interference between Human HBEGF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 91-105 | 97 |
| EDTA plasma (n=8) | 92-106 | 99 |
| Cell culture media (n=8) | 85-96 | 90 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 47.57 | 114.86 | 444.51 | 43.48 | 117.12 | 469.09 |
| Standard deviation | 2.46 | 5.25 | 14.36 | 2.75 | 5.36 | 24.81 |
| CV (%) | 5.18 | 4.57 | 3.23 | 6.33 | 4.58 | 5.29 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
