| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.94ng/mL |
| Detection Limit: |
1.56~100ng/mL |
| Short Description: |
This HB Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
HBB |
| Gene ID: |
3043 |
| Uniprot ID: |
HBB_HUMAN |
| Specificity: |
This kit recognizes Human HB in samples. No significant cross-reactivity or interference between Human HB and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Glucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus. S-nitrosylated.a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2). Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure. |
| Function | Involved in oxygen transport from the lung to the various peripheral tissues. LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure. Spinorphin: Functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation. |
| Protein Name | Hemoglobin Subunit BetaBeta-GlobinHemoglobin Beta Chain Cleaved Into - Lvv-Hemorphin-7 - Spinorphin |
| Database Links | Reactome: R-HSA-1237044Reactome: R-HSA-1247673Reactome: R-HSA-2168880Reactome: R-HSA-6798695Reactome: R-HSA-9613829Reactome: R-HSA-9615710Reactome: R-HSA-9707564Reactome: R-HSA-9707616Reactome: R-HSA-983231 |
| Alternative ELISA Names | Hemoglobin Subunit Beta ELISA kitBeta-Globin ELISA kitHemoglobin Beta Chain Cleaved Into - Lvv-Hemorphin-7 - Spinorphin ELISA kitHBB ELISA kit |
| Specificity | This kit recognizes Human HB in samples. No significant cross-reactivity or interference between Human HB and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-106 | 99 |
| EDTA plasma (n=8) | 86-101 | 93 |
| Cell culture media (n=8) | 92-105 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 5.50 | 14.10 | 49.30 | 5.00 | 12.80 | 50.10 |
| Standard deviation | 0.30 | 0.60 | 1.80 | 0.30 | 0.50 | 2.70 |
| CV (%) | 5.45 | 4.26 | 3.65 | 6.00 | 3.91 | 5.39 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
