| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69pg/mL |
| Detection Limit: |
7.81~500pg/mL |
| Short Description: |
This HAVCR1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
HAVCR1 |
| Gene ID: |
26762 |
| Uniprot ID: |
HAVR1_HUMAN |
| Specificity: |
This kit recognizes Human KIM-1 in samples. No significant cross-reactivity or interference between Human KIM-1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Ubiquitinated at two lysine residues Lys-338 and Lys-346 on its cytoplasmic domain. Ubiquitination promotes receptor endocytosis and target receptors for lysosomal degradation and termination of receptor signaling. (Microbial infection) Ubiquitination is required for Dengue virus endocytosis. |
| Function | Phosphatidylserine receptor that plays an important functional role in regulatory B-cells homeostasis including generation, expansion and suppressor functions. As P-selectin/SELPLG ligand, plays a specialized role in activated but not naive T-cell trafficking during inflammatory responses. Controls thereby T-cell accumulation in the inflamed central nervous system (CNS) and the induction of autoimmune disease. Regulates also expression of various anti-inflammatory cytokines and co-inhibitory ligands including IL10. Acts as regulator of T-cell proliferation. May play a role in kidney injury and repair. (Microbial infection) Acts as a receptor for Hepatitis A virus. (Microbial infection) Acts as a receptor for Ebolavirus and Marburg virus by binding exposed phosphatidyl-serine at the surface of virion membrane. Serves as a dual receptor for Ebolavirus by also interacting with envelope glycoprotein GP. (Microbial infection) Acts as a receptor for Dengue virus by binding exposed phosphatidyl-serine at the surface of virion membrane. TIM1 and Dengue virus are co-internalized during virus entry. (Microbial infection) Acts as a receptor for Zika virus by binding to envelope protein E. (Microbial infection) Plays a positive role in Chikungunya virus cell entry. |
| Protein Name | Hepatitis A Virus Cellular Receptor 1Havcr-1Kidney Injury Molecule 1Kim-1T-Cell Immunoglobulin And Mucin Domain-Containing Protein 1Timd-1T-Cell Immunoglobulin Mucin Receptor 1TimTim-1T-Cell Membrane Protein 1Cd Antigen Cd365 |
| Database Links | Reactome: R-HSA-9694614 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative ELISA Names | Hepatitis A Virus Cellular Receptor 1 ELISA kitHavcr-1 ELISA kitKidney Injury Molecule 1 ELISA kitKim-1 ELISA kitT-Cell Immunoglobulin And Mucin Domain-Containing Protein 1 ELISA kitTimd-1 ELISA kitT-Cell Immunoglobulin Mucin Receptor 1 ELISA kitTim ELISA kitTim-1 ELISA kitT-Cell Membrane Protein 1 ELISA kitCd Antigen Cd365 ELISA kitHAVCR1 ELISA kitKIM1 ELISA kitTIM1 ELISA kitTIMD1 ELISA kit |
| Specificity | This kit recognizes Human KIM-1 in samples. No significant cross-reactivity or interference between Human KIM-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-98 | 92 |
| EDTA plasma (n=8) | 89-102 | 94 |
| Cell culture media (n=8) | 93-109 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 24.94 | 61.85 | 217.85 | 23.48 | 57.68 | 231.74 |
| Standard deviation | 1.40 | 2.92 | 8.78 | 1.24 | 2.64 | 12.14 |
| CV (%) | 5.61 | 4.72 | 4.03 | 5.28 | 4.58 | 5.24 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
