• Human HAP1 (Huntingtin Associated Protein 1) Sandwich ELISA Kit (STJE0009389)

Human HAP1 (Huntingtin Associated Protein 1) Sandwich ELISA Kit (STJE0009389)

SKU:
STJE0009389

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.113ng/mL
Detection Limit: 0.312-20ng/mL
Short Description: This HAP1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: HAP1
Gene ID: 9001
Uniprot ID: HAP1_HUMAN
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity Predominantly expressed in brain. Selectively expressed in neurons.
Post Translational Modifications
Function Originally identified as neuronal protein that specifically associates with HTT/huntingtin and the binding is enhanced by an expanded polyglutamine repeat within HTT possibly affecting HAP1 interaction properties. Both HTT and HAP1 are involved in intracellular trafficking and HAP1 is proposed to link HTT to motor proteins and/or transport cargos. Seems to play a role in vesicular transport within neurons and axons such as from early endosomes to late endocytic compartments and to promote neurite outgrowth. The vesicular transport function via association with microtubule-dependent transporters can be attenuated by association with mutant HTT. Involved in the axonal transport of BDNF and its activity-dependent secretion.the function seems to involve HTT, DCTN1 and a complex with SORT1. Involved in APP trafficking and seems to facilitate APP anterograde transport and membrane insertion thereby possibly reducing processing into amyloid beta. Involved in delivery of gamma-aminobutyric acid (GABA(A)) receptors to synapses.the function is dependent on kinesin motor protein KIF5 and is disrupted by HTT with expanded polyglutamine repeat. Involved in regulation of autophagosome motility by promoting efficient retrograde axonal transport. Seems to be involved in regulation of membrane receptor recycling and degradation, and respective signal transduction, including GABA(A) receptors, tyrosine kinase receptors, EGFR, IP3 receptor and androgen receptor. Among others suggested to be involved in control of feeding behavior (involving hypothalamic GABA(A) receptors), cerebellar and brainstem development (involving AHI1 and NTRK1/TrkA), postnatal neurogenesis (involving hypothalamic NTRK2/TrkB), and ITPR1/InsP3R1-mediated Ca(2+) release (involving HTT and possibly the effect of mutant HTT). Via association with DCTN1/dynactin p150-glued and HTT/huntingtin involved in cytoplasmic retention of REST in neurons. May be involved in ciliogenesis. Involved in regulation of exocytosis. Seems to be involved in formation of cytoplasmic inclusion bodies (STBs). In case of anomalous expression of TBP, can sequester a subset of TBP into STBs.sequestration is enhanced by an expanded polyglutamine repeat within TBP. HAP1-containing STBs have been proposed to play a protective role against neurodegeneration in Huntigton disease (HD) and spinocerebellar ataxia 17 (SCA17).
Protein Name Huntingtin-Associated Protein 1
Hap-1
Neuroan 1
Database Links
Cellular Localisation Cytoplasm
Cell Projection
Axon
Presynapse
Cytoskeleton
Dendritic Spine
Dendrite
Lysosome
Endoplasmic Reticulum
Mitochondrion
Nucleus
Cytoplasmic Vesicle
Autophagosome
Early Endosome
Growth Cone
Neuron Projection
Secretory Vesicle
Synaptic Vesicle
Localizes To Large Nonmembrane-Bound Cytoplasmic Bodies Found In Various Types Of Neurons
Called Stigmoid Bodies (Stbs)
Localization To Neuronal Processes And Neurite Tips Is Decreased By Ywhaz
In The Nucleus Localizes To Nuclear Rods
Alternative ELISA Names Huntingtin-Associated Protein 1 ELISA kit
Hap-1 ELISA kit
Neuroan 1 ELISA kit
HAP1 ELISA kit
HAP2 ELISA kit
HLP1 ELISA kit
output

Information sourced from Uniprot.org

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