• Human HAase (Hyaluronidase) Sandwich ELISA (STJE0003526)

Human HAase (Hyaluronidase) Sandwich ELISA (STJE0003526)

SKU:
STJE0003526

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 1.88ng/mL
Detection Limit: 3.13~200ng/mL
Short Description: This HAase Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: HYAL1
Gene ID: 3373
Uniprot ID: HYAL1_HUMAN
Specificity: This kit recognizes Human HAase in samples. No significant cross-reactivity or interference between Human HAase and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth.
Protein Name Hyaluronidase-1
Hyal-1
Hyaluronoglucosaminidase-1
Lung Carcinoma Protein 1
Luca-1
Database Links Reactome: R-HSA-2024101
Reactome: R-HSA-2160916
Reactome: R-HSA-2206280
Cellular Localisation Secreted
Lysosome
Alternative ELISA Names Hyaluronidase-1 ELISA kit
Hyal-1 ELISA kit
Hyaluronoglucosaminidase-1 ELISA kit
Lung Carcinoma Protein 1 ELISA kit
Luca-1 ELISA kit
HYAL1 ELISA kit
LUCA1 ELISA kit
Specificity This kit recognizes Human HAase in samples. No significant cross-reactivity or interference between Human HAase and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-110 102
EDTA plasma (n=8) 87-100 94
Cell culture media (n=8) 94-107 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 10.09 31.24 82.97 10.60 32.43 80.64
Standard deviation 0.63 1.80 3.39 0.72 1.89 2.69
CV (%) 6.24 5.76 4.09 6.79 5.83 3.34
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance