• Human GUSB (Beta-glucuronidase) Sandwich ELISA (STJE0002650)
  • Human GUSB (Beta-glucuronidase) Sandwich ELISA (STJE0002650)

Human GUSB (Beta-glucuronidase) Sandwich ELISA (STJE0002650)

SKU:
STJE0002650

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This GUSB Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: GUSB
Gene ID: 2990
Uniprot ID: BGLR_HUMAN
Specificity: This kit recognizes Human GUS Beta in samples. No significant cross-reactivity or interference between Human GUS Beta and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Plays an important role in the degradation of dermatan and keratan sulfates.
Protein Name Beta-Glucuronidase
Beta-G1
Database Links Reactome: R-HSA-2024096
Reactome: R-HSA-2160916
Reactome: R-HSA-2206292
Reactome: R-HSA-6798695
Cellular Localisation Lysosome
Alternative ELISA Names Beta-Glucuronidase ELISA kit
Beta-G1 ELISA kit
GUSB ELISA kit
Specificity This kit recognizes Human GUSβ in samples. No significant cross-reactivity or interference between Human GUSβ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-102 93
EDTA plasma (n=8) 90-100 95
Cell culture media (n=8) 93-109 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.47 1.29 4.89 0.52 1.31 5.13
Standard deviation 0.03 0.05 0.25 0.03 0.06 0.17
CV (%) 6.38 3.88 5.11 5.77 4.58 3.31
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance