| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This human GTF2A1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
GTF2A1 |
| Gene ID: |
2957 |
| Uniprot ID: |
TF2AA_HUMAN |
| Specificity: |
This kit recognizes Human GTF2A1 in samples. No significant cross-reactivity or interference between Human GTF2A1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The alpha and beta subunits are postranslationally produced from the precursor form by TASP1. The cleavage promotes proteasomal degradation. |
| Function | TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. |
| Protein Name | Transcription Initiation Factor Iia Subunit 1General Transcription Factor Iia Subunit 1TfiialTranscription Initiation Factor Tfiia 42 Kda SubunitTfiia-42 Cleaved Into - Transcription Initiation Factor Iia Alpha ChainTfiia P35 Subunit - Transcription Initiation Factor Iia Beta ChainTfiia P19 Subunit |
| Database Links | Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-674695Reactome: R-HSA-6807505Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-75953Reactome: R-HSA-76042Reactome: R-HSA-9018519 |
| Cellular Localisation | Nucleus |
| Alternative CLIA Names | Transcription Initiation Factor Iia Subunit 1 CLIA kitGeneral Transcription Factor Iia Subunit 1 CLIA kitTfiial CLIA kitTranscription Initiation Factor Tfiia 42 Kda Subunit CLIA kitTfiia-42 Cleaved Into - Transcription Initiation Factor Iia Alpha Chain CLIA kitTfiia P35 Subunit - Transcription Initiation Factor Iia Beta Chain CLIA kitTfiia P19 Subunit CLIA kitGTF2A1 CLIA kitTF2A1 CLIA kit |
| Specificity | This kit recognizes Human GTF2A1 in samples. No significant cross-reactivity or interference between Human GTF2A1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-105 | 97 |
| EDTA plasma(n=8) | 99-119 | 108 |
| Cell culture media(n=8) | 97-111 | 105 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 276.73 | 541.51 | 2290.84 | 294.81 | 591.43 | 2151.34 |
| Standard deviation | 26.73 | 54.58 | 175.48 | 36.26 | 43.06 | 212.98 |
| CV (%) | 9.66 | 10.08 | 7.66 | 12.30 | 7.28 | 9.90 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
