Human Growth hormone receptor/GHR protein (Recombinant) (C-hFc & His) (STJP002605)

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STJP002605-100
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Host: HEK293 cells
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Formulation: Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Determination Method: < 0.01 EU/Mu g of the protein by LAL method.
Immunoreactivity: Measured by its binding ability in a functional ELISA. Immobilized Human GH at 2 Mu g/mL (100 Mu L/well) can bind Human GHR with a linear range of 0.1-8.58 ng/mL.
Gene Symbol: GHR
Gene ID: 2690
Uniprot ID: GHR_HUMAN
Immunogen Region: Ala27-Tyr264
Immunogen: Recombinant Human Growth hormone receptor/GHR Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala 27-Tyr 264) of human Growth Hormone Receptor (GHR) (Accession #NP_000154.1) fused with an Fc, 6×His tag
Immunogen Sequence: AILSRAPWSLQSVNPGLKTN SSKEPKFTKCRSPERETFSC HWTDEVHHGTKNLGPIQLFY TRRNTQEWTQEWKECPDYVS AGENSCYFNSSFTSIWIPYC IKLTSNGGTVDEKCFSVDEI VQPDPPIALNWTLLNVSLTG IHADIQVRWEAPRNADIQKG WMVLEYELQYKEVNETKWKM MDPILTTSVPVYSLKVDKEY EVRVRSKQRNSGNYGEFSEV LYVTLPQMSQFTCEEDFY
Post Translational Modifications The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17. On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2. Ubiquitinated by the ECS(SOCS2) complex following ligand-binding and phosphorylation by JAK2, leading to its degradation by the proteasome. Regulation by the ECS(SOCS2) complex acts as a negative feedback loop of growth hormone receptor signaling. Ubiquitination is not sufficient for GHR internalization.
Function Receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. Growth hormone-binding protein: The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. Isoform 2: Up-regulates the production of the soluble Growth hormone-binding protein form (GHBP) and acts as a negative inhibitor of growth hormone signaling.
Protein Name Growth Hormone Receptor
Gh Receptor
Somatotropin Receptor Cleaved Into - Growth Hormone-Binding Protein
Gh-Binding Protein
Ghbp
Serum-Binding Protein
Database Links Reactome: R-HSA-1170546
Reactome: R-HSA-982772
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
On Growth Hormone Binding
Ghr Is Ubiquitinated
Internalized
Down-Regulated And Transported Into A Degradative Or Non-Degradative Pathway
Isoform 2: Cell Membrane
Remains Fixed To The Cell Membrane And Is Not Internalized
Growth Hormone-Binding Protein: Secreted
Complexed To A Substantial Fraction Of Circulating Gh
Alternative Protein Names Growth Hormone Receptor protein
Gh Receptor protein
Somatotropin Receptor Cleaved Into - Growth Hormone-Binding Protein protein
Gh-Binding Protein protein
Ghbp protein
Serum-Binding Protein protein
GHR protein

Information sourced from Uniprot.org

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