• Human GREM1 (Gremlin-1) Sandwich ELISA (STJE0002902)
  • Human GREM1 (Gremlin-1) Sandwich ELISA (STJE0002902)

Human GREM1 (Gremlin-1) Sandwich ELISA (STJE0002902)

SKU:
STJE0002902

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This GREM1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: GREM1
Gene ID: 26585
Uniprot ID: GREM1_HUMAN
Specificity: This kit recognizes Human GREM1 in samples. No significant cross-reactivity or interference between Human GREM1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Antagonist of BMP2.inhibits BMP2-mediated differentiation of osteoblasts (in vitro). Acts as inhibitor of monocyte chemotaxis. Can inhibit the growth or viability of normal cells but not transformed cells when is overexpressed.
Protein Name Gremlin-1
Cell Proliferation-Inducing Gene 2 Protein
Cysteine Knot Superfamily 1 - Bmp Antagonist 1
Dan Domain Family Member 2
Down-Regulated In Mos-Transformed Cells Protein
Increased In High Glucose Protein 2
Ihg-2
Cellular Localisation Secreted
Alternative ELISA Names Gremlin-1 ELISA kit
Cell Proliferation-Inducing Gene 2 Protein ELISA kit
Cysteine Knot Superfamily 1 - Bmp Antagonist 1 ELISA kit
Dan Domain Family Member 2 ELISA kit
Down-Regulated In Mos-Transformed Cells Protein ELISA kit
Increased In High Glucose Protein 2 ELISA kit
Ihg-2 ELISA kit
GREM1 ELISA kit
CKTSF1B1 ELISA kit
DAND2 ELISA kit
DRM ELISA kit
PIG2 ELISA kit
Specificity This kit recognizes Human GREM1 in samples. No significant cross-reactivity or interference between Human GREM1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-97 90
EDTA plasma (n=8) 89-102 97
Cell culture media (n=8) 88-101 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.47 1.43 4.34 0.45 1.38 4.48
Standard deviation 0.03 0.06 0.13 0.03 0.08 0.24
CV (%) 6.38 4.20 3.00 6.67 5.80 5.36
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance