| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human GPRC5A kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
GPRC5A |
| Gene ID: |
9052 |
| Uniprot ID: |
RAI3_HUMAN |
| Specificity: |
This kit recognizes Human GPRC5A in samples. No significant cross-reactivity or interference between Human GPRC5A and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed at high level in fetal and adult lung tissues but repressed in most human lung cancers. Constitutively expressed in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal cord at low to moderate levels. Not detectable in fetal heart, brain, and liver and adult heart, brain, liver, skeletal muscle, pancreas, spleen, thymus, and peripheral leukocytes. expressed at low but detectable level in pancreas and heart. |
| Post Translational Modifications | Phosphorylated in two conserved double-tyrosine motifs, Tyr-317/Tyr-320 and Tyr-347/Tyr-350, by EGFR.leading to inactivation of the tumor suppressive function of GPRC5A in lung cancer cells. Tyr-317 and Tyr-320 are the preferred residues responsible for EGFR-mediated GPRC5A phosphorylation. |
| Function | Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling. May act as a lung tumor suppressor. |
| Protein Name | Retinoic Acid-Induced Protein 3G-Protein Coupled Receptor Family C Group 5 Member APhorbol Ester Induced Gene 1Peig-1Retinoic Acid-Induced Gene 1 ProteinRaig-1 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCytoplasmic Vesicle MembraneLocalized In Perinuclear VesiclesProbably Golgi-Associated Vesicles |
| Alternative CLIA Names | Retinoic Acid-Induced Protein 3 CLIA kitG-Protein Coupled Receptor Family C Group 5 Member A CLIA kitPhorbol Ester Induced Gene 1 CLIA kitPeig-1 CLIA kitRetinoic Acid-Induced Gene 1 Protein CLIA kitRaig-1 CLIA kitGPRC5A CLIA kitGPCR5A CLIA kitRAI3 CLIA kitRAIG1 CLIA kit |
| Specificity | This kit recognizes Human GPRC5A in samples. No significant cross-reactivity or interference between Human GPRC5A and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-108 | 101 |
| EDTA plasma(n=8) | 84-96 | 90 |
| Cell culture media(n=8) | 93-108 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 188.24 | 419.40 | 1399.72 | 188.31 | 452.08 | 1309.06 |
| Standard deviation | 21.33 | 31.71 | 93.22 | 15.35 | 48.55 | 125.80 |
| CV (%) | 11.33 | 7.56 | 6.66 | 8.15 | 10.74 | 9.61 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
