• Human GPI (Glucose-6-phosphate isomerase) CLIA Kit (STJC0000795)

Human GPI (Glucose-6-phosphate isomerase) CLIA Kit (STJC0000795)

SKU:
STJC0000795

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human GPI kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: GPI
Gene ID: 2821
Uniprot ID: G6PI_HUMAN
Specificity: This kit recognizes Human GPI in samples. No significant cross-reactivity or interference between Human GPI and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway. ISGylated.
Function In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.
Protein Name Glucose-6-Phosphate Isomerase
Gpi
Autocrine Motility Factor
Amf
Neuroleukin
Nlk
Phosphoglucose Isomerase
Pgi
Phosphohexose Isomerase
Phi
Sperm Antigen 36
Sa-36
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-6798695
Reactome: R-HSA-70171
Reactome: R-HSA-70263
Cellular Localisation Cytoplasm
Secreted
Alternative CLIA Names Glucose-6-Phosphate Isomerase CLIA kit
Gpi CLIA kit
Autocrine Motility Factor CLIA kit
Amf CLIA kit
Neuroleukin CLIA kit
Nlk CLIA kit
Phosphoglucose Isomerase CLIA kit
Pgi CLIA kit
Phosphohexose Isomerase CLIA kit
Phi CLIA kit
Sperm Antigen 36 CLIA kit
Sa-36 CLIA kit
GPI CLIA kit
Specificity This kit recognizes Human GPI in samples. No significant cross-reactivity or interference between Human GPI and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-116 106
EDTA plasma(n=8) 89-103 95
Cell culture media(n=8) 97-111 104

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 49.63 100.91 367.25 50.25 92.18 370.12
Standard deviation 4.70 10.77 37.83 5.41 6.45 22.39
CV (%) 9.47 10.67 10.30 10.77 7.00 6.05
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance