| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.12 ng/mL |
| Detection Limit: |
0.31-20 ng/mL |
| Short Description: |
This gp130 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
IL6ST |
| Gene ID: |
3572 |
| Uniprot ID: |
IL6RB_HUMAN |
| Specificity: |
This kit recognizes Human gp130 in samples. No significant cross-reactivity or interference between Human gp130 and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. Isoform 2: Expressed in blood serum (at protein level). |
| Post Translational Modifications | Phosphorylation of Ser-782 down-regulates cell surface expression. Heavily N-glycosylated. Glycosylation is required for protein stability and localization in plasma membrane but not for ligand binding. |
| Function | Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates the intracellular JAK-MAPK and JAK-STAT3 signaling pathways. That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3. In parallel, the IL6 signaling pathway induces the expression of two cytokine receptor signaling inhibitors, SOCS1 and SOCS3, which inhibit JAK and terminate the activity of the IL6 signaling pathway as a negative feedback loop. Also activates the yes-associated protein 1 (YAP) and NOTCH pathways to control inflammation-induced epithelial regeneration, independently of STAT3. Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL27RA/WSX1 and CNTFR. Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism. Has a role in embryonic development. Essential for survival of motor and sensory neurons and for differentiation of astrocytes. Required for expression of TRPA1 in nociceptive neurons. Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation. Required for normal trabecular bone mass and cortical bone composition. Isoform 2: Binds to the soluble IL6:sIL6R complex (hyper-IL6), thereby blocking IL6 trans-signaling. Inhibits sIL6R-dependent acute phase response. Also blocks IL11 cluster signaling through IL11R. |
| Protein Name | Interleukin-6 Receptor Subunit BetaIl-6 Receptor Subunit BetaIl-6r Subunit BetaIl-6r-BetaIl-6rbCdw130Interleukin-6 Signal TransducerMembrane Glycoprotein 130Gp130Oncostatin-M Receptor Subunit AlphaCd Antigen Cd130 |
| Database Links | Reactome: R-HSA-1059683Reactome: R-HSA-110056 P40189-1Reactome: R-HSA-112411 P40189-1Reactome: R-HSA-6788467Reactome: R-HSA-8984722Reactome: R-HSA-9020956 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: Secreted |
| Alternative ELISA Names | Interleukin-6 Receptor Subunit Beta ELISA kitIl-6 Receptor Subunit Beta ELISA kitIl-6r Subunit Beta ELISA kitIl-6r-Beta ELISA kitIl-6rb ELISA kitCdw130 ELISA kitInterleukin-6 Signal Transducer ELISA kitMembrane Glycoprotein 130 ELISA kitGp130 ELISA kitOncostatin-M Receptor Subunit Alpha ELISA kitCd Antigen Cd130 ELISA kitIL6ST ELISA kit |
| Specificity | This kit recognizes Human gp130 in samples. No significant cross-reactivity or interference between Human gp130 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-110 | 102 |
| EDTA plasma (n=8) | 90-102 | 95 |
| Cell culture media (n=8) | 89-102 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.97 | 3.10 | 8.84 | 0.89 | 3.06 | 8.03 |
| Standard deviation | 0.06 | 0.14 | 0.33 | 0.06 | 0.15 | 0.43 |
| CV (%) | 6.46 | 4.63 | 3.75 | 6.56 | 4.89 | 5.32 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
