• Human GLP-2 (Glucagon Like Peptide 2) Competitive ELISA (STJE0003551)
  • Human GLP-2 (Glucagon Like Peptide 2) Competitive ELISA (STJE0003551)

Human GLP-2 (Glucagon Like Peptide 2) Competitive ELISA (STJE0003551)

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STJE0003551

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This GLP-2 Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Gene Symbol: GCG
Gene ID: 2641
Uniprot ID: GLUC_HUMAN
Specificity: This kit recognizes Human GLP-2 in samples. No significant cross-reactivity or interference between Human GLP-2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
Function Glucagon: Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon-like peptide 1: Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6. Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis (Probable). Glucagon-like peptide 2: Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability. Oxyntomodulin: Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness. Glicentin: May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
Protein Name Pro-Glucagon Cleaved Into - Glicentin - Glicentin-Related Polypeptide
Grpp - Oxyntomodulin
Oxm
Oxy - Glucagon - Glucagon-Like Peptide 1
Glp-1
Incretin Hormone - Glucagon-Like Peptide 1(7-37
Glp-1(7-37 - Glucagon-Like Peptide 1(7-36
Glp-1(7-36 - Glucagon-Like Peptide 2
Glp-2
Database Links Reactome: R-HSA-163359
Reactome: R-HSA-381676
Reactome: R-HSA-381771
Reactome: R-HSA-416476
Reactome: R-HSA-418555
Reactome: R-HSA-420092
Reactome: R-HSA-422085
Cellular Localisation Secreted
Glucagon-Like Peptide 1: Secreted
Alternative ELISA Names Pro-Glucagon Cleaved Into - Glicentin - Glicentin-Related Polypeptide ELISA kit
Grpp - Oxyntomodulin ELISA kit
Oxm ELISA kit
Oxy - Glucagon - Glucagon-Like Peptide 1 ELISA kit
Glp-1 ELISA kit
Incretin Hormone - Glucagon-Like Peptide 1(7-37 ELISA kit
Glp-1(7-37 - Glucagon-Like Peptide 1(7-36 ELISA kit
Glp-1(7-36 - Glucagon-Like Peptide 2 ELISA kit
Glp-2 ELISA kit
GCG ELISA kit
Specificity This kit recognizes GLP-2 in samples. No significant cross-reactivity or interference between GLP-2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-101 93
EDTA plasma(n=8) 90-105 97
Cell culture media(n=8) 94-105 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.50 1.10 4.20 0.50 1.00 4.30
Standard deviation 0.03 0.05 0.21 0.03 0.04 0.20
CV (%) 6.00 4.55 5.00 6.00 4.00 4.65
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance