• Human GLI1 (GLI Family Zinc Finger Protein 1) Sandwich ELISA Kit (STJE0009186)

Human GLI1 (GLI Family Zinc Finger Protein 1) Sandwich ELISA Kit (STJE0009186)

SKU:
STJE0009186

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.122ng/mL
Detection Limit: 0.312-20ng/mL
Short Description: This GLI1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: GLI1
Gene ID: 2735
Uniprot ID: GLI1_HUMAN
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity Detected in testis (at protein level). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta.isoform 2 is not detectable in these tissues.
Post Translational Modifications Phosphorylated in vitro by ULK3. Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1. Ubiquitinated by the CRL2(FEM1B) complex, suppressing GLI1 transcriptional activator activity.
Function Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling. Isoform 2: Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration.
Protein Name Zinc Finger Protein Gli1
Glioma-Associated Oncogene
Oncogene Gli
Database Links Reactome: R-HSA-5610780
Reactome: R-HSA-5610787
Reactome: R-HSA-5632684
Reactome: R-HSA-5635851
Cellular Localisation Cytoplasm
Nucleus
Tethered In The Cytoplasm By Binding To Sufu
Activation And Translocation To The Nucleus Is Promoted By Interaction With Stk36
Phosphorylation By Ulk3 May Promote Nuclear Localization
Translocation To The Nucleus Is Promoted By Interaction With Zic1
Isoform 2: Cytoplasm
Alternative ELISA Names Zinc Finger Protein Gli1 ELISA kit
Glioma-Associated Oncogene ELISA kit
Oncogene Gli ELISA kit
GLI1 ELISA kit
GLI ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance