• Human GLA (Alpha-galactosidase A) CLIA Kit (STJC0000478)

Human GLA (Alpha-galactosidase A) CLIA Kit (STJC0000478)

SKU:
STJC0000478

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human GLA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: GLA
Gene ID: 2717
Uniprot ID: AGAL_HUMAN
Specificity: This kit recognizes Human GL Alpha in samples. No significant cross-reactivity or interference between Human GL Alpha and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Catalyzes the hydrolysis of glycosphingolipids and participates in their degradation in the lysosome.
Protein Name Alpha-Galactosidase A
Alpha-D-Galactosidase A
Alpha-D-Galactoside Galactohydrolase
Galactosylgalactosylglucosylceramidase Gla
Melibiase
Agalsidase
Database Links Reactome: R-HSA-1660662
Reactome: R-HSA-6798695
Cellular Localisation Lysosome
Alternative CLIA Names Alpha-Galactosidase A CLIA kit
Alpha-D-Galactosidase A CLIA kit
Alpha-D-Galactoside Galactohydrolase CLIA kit
Galactosylgalactosylglucosylceramidase Gla CLIA kit
Melibiase CLIA kit
Agalsidase CLIA kit
GLA CLIA kit
Specificity This kit recognizes Human GLα in samples. No significant cross-reactivity or interference between Human GLα and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-114 104
EDTA plasma(n=8) 90-100 95
Cell culture media(n=8) 88-104 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 52.48 111.55 336.34 50.91 117.29 331.85
Standard deviation 6.57 13.02 22.10 5.03 12.17 35.64
CV (%) 12.52 11.67 6.57 9.88 10.38 10.74
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance