| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human GKN1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
GKN1 |
| Gene ID: |
56287 |
| Uniprot ID: |
GKN1_HUMAN |
| Specificity: |
This kit recognizes Human GKN1 in samples. No significant cross-reactivity or interference between Human GKN1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in stomach (at protein level). No expression is detected in cancer tissue or gastric cancer cell lines. |
| Function | Has mitogenic activity and may be involved in maintaining the integrity of the gastric mucosal epithelium. |
| Protein Name | Gastrokine-118 Kda Antrum Mucosa ProteinAmp-18Protein Ca11 |
| Cellular Localisation | SecretedCytoplasmic GranuleGolgi ApparatusShows Abundant Granular Cytoplasmic StainingWith Perinuclear Accentuation Suggestive Of The Golgi Apparatus |
| Alternative CLIA Names | Gastrokine-1 CLIA kit18 Kda Antrum Mucosa Protein CLIA kitAmp-18 CLIA kitProtein Ca11 CLIA kitGKN1 CLIA kitAMP18 CLIA kitCA11 CLIA kitUNQ489 CLIA kitPRO1005 CLIA kit |
| Specificity | This kit recognizes Human GKN1 in samples. No significant cross-reactivity or interference between Human GKN1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-101 | 95 |
| EDTA plasma(n=8) | 89-100 | 94 |
| Cell culture media(n=8) | 92-107 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 105.80 | 195.06 | 755.20 | 111.99 | 207.88 | 690.43 |
| Standard deviation | 9.54 | 19.97 | 79.07 | 11.41 | 17.34 | 65.45 |
| CV (%) | 9.02 | 10.24 | 10.47 | 10.19 | 8.34 | 9.48 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
