Human GHR (Growth hormone receptor) Sandwich ELISA (STJE0003461)

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STJE0003461

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.94ng/mL
Detection Limit: 1.56~100ng/mL
Short Description: This GHR Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: GHR
Gene ID: 2690
Uniprot ID: GHR_HUMAN
Specificity: This kit recognizes Human GHR in samples. No significant cross-reactivity or interference between Human GHR and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17. On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2. On ligand binding, ubiquitinated on lysine residues in the cytoplasmic domain. This ubiquitination is not sufficient for GHR internalization.
Function Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling.
Protein Name Growth Hormone Receptor
Gh Receptor
Somatotropin Receptor Cleaved Into - Growth Hormone-Binding Protein
Gh-Binding Protein
Ghbp
Serum-Binding Protein
Database Links Reactome: R-HSA-1170546
Reactome: R-HSA-982772
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
On Growth Hormone Binding
Ghr Is Ubiquitinated
Internalized
Down-Regulated And Transported Into A Degradative Or Non-Degradative Pathway
Isoform 2: Cell Membrane
Remains Fixed To The Cell Membrane And Is Not Internalized
Growth Hormone-Binding Protein: Secreted
Complexed To A Substantial Fraction Of Circulating Gh
Alternative ELISA Names Growth Hormone Receptor ELISA kit
Gh Receptor ELISA kit
Somatotropin Receptor Cleaved Into - Growth Hormone-Binding Protein ELISA kit
Gh-Binding Protein ELISA kit
Ghbp ELISA kit
Serum-Binding Protein ELISA kit
GHR ELISA kit
Specificity This kit recognizes Human GHR in samples. No significant cross-reactivity or interference between Human GHR and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-96 90
EDTA plasma (n=8) 95-109 102
Cell culture media (n=8) 93-111 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 5.40 12.54 38.08 5.76 13.64 38.32
Standard deviation 0.37 0.56 1.94 0.31 0.71 1.36
CV (%) 6.85 4.47 5.09 5.38 5.21 3.55
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance