Applications: |
CLIA |
Reactivity: |
Human |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
9.38pg/mL |
Detection Limit: |
15.63~1000pg/mL |
Short Description: |
This human GDNF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Chemiluminescence |
Gene Symbol: |
GDNF |
Gene ID: |
2668 |
Uniprot ID: |
GDNF_HUMAN |
Specificity: |
This kit recognizes Human GDNF in samples. No significant cross-reactivity or interference between Human GDNF and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Tissue Specificity | In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change. |
Function | Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. |
Protein Name | Glial Cell Line-Derived Neurotrophic FactorHgdnfAstrocyte-Derived Trophic FactorAtf |
Database Links | Reactome: R-HSA-419037Reactome: R-HSA-5673001Reactome: R-HSA-8853659 |
Cellular Localisation | Secreted |
Alternative CLIA Names | Glial Cell Line-Derived Neurotrophic Factor CLIA kitHgdnf CLIA kitAstrocyte-Derived Trophic Factor CLIA kitAtf CLIA kitGDNF CLIA kit |
Specificity | This kit recognizes Human GDNF in samples. No significant cross-reactivity or interference between Human GDNF and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 86-101 | 93 |
EDTA plasma(n=8) | 91-105 | 96 |
Cell culture media(n=8) | 88-100 | 94 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 48.89 | 111.48 | 476.63 | 53.07 | 112.07 | 448.71 |
Standard deviation | 5.55 | 7.99 | 32.03 | 5.58 | 11.99 | 49.99 |
CV (%) | 11.35 | 7.17 | 6.72 | 10.51 | 10.70 | 11.14 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance