Applications: | ELISA |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Sensitivity: | 0.064ng/mL |
Detection Limit: | 0.156-10ng/mL |
Short Description: | This GDF5 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
Assay Time: | 4.5 hrs |
Gene Symbol: | GDF5 |
Gene ID: | 8200 |
Uniprot ID: | GDF5_HUMAN |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Tissue Specificity | Predominantly expressed in long bones during embryonic development. Expressed in monocytes (at protein level). |
Post Translational Modifications | |
Function | Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction. Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG. Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. |
Protein Name | Growth/Differentiation Factor 5 Gdf-5 Bone Morphogenetic Protein 14 Bmp-14 Cartilage-Derived Morphogenetic Protein 1 Cdmp-1 Lipopolysaccharide-Associated Protein 4 Lap-4 Lps-Associated Protein 4 Radotermin |
Database Links | Reactome: R-HSA-2129379 |
Cellular Localisation | Secreted Cell Membrane |
Alternative ELISA Names | Growth/Differentiation Factor 5 ELISA kit Gdf-5 ELISA kit Bone Morphogenetic Protein 14 ELISA kit Bmp-14 ELISA kit Cartilage-Derived Morphogenetic Protein 1 ELISA kit Cdmp-1 ELISA kit Lipopolysaccharide-Associated Protein 4 ELISA kit Lap-4 ELISA kit Lps-Associated Protein 4 ELISA kit Radotermin ELISA kit GDF5 ELISA kit BMP14 ELISA kit CDMP1 ELISA kit |
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Information sourced from Uniprot.org