| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.064ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This GDF5 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
GDF5 |
| Gene ID: |
8200 |
| Uniprot ID: |
GDF5_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Predominantly expressed in long bones during embryonic development. Expressed in monocytes (at protein level). |
| Post Translational Modifications | |
| Function | Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction. Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG. Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. |
| Protein Name | Growth/Differentiation Factor 5Gdf-5Bone Morphogenetic Protein 14Bmp-14Cartilage-Derived Morphogenetic Protein 1Cdmp-1Lipopolysaccharide-Associated Protein 4Lap-4Lps-Associated Protein 4Radotermin |
| Database Links | Reactome: R-HSA-2129379 |
| Cellular Localisation | SecretedCell Membrane |
| Alternative ELISA Names | Growth/Differentiation Factor 5 ELISA kitGdf-5 ELISA kitBone Morphogenetic Protein 14 ELISA kitBmp-14 ELISA kitCartilage-Derived Morphogenetic Protein 1 ELISA kitCdmp-1 ELISA kitLipopolysaccharide-Associated Protein 4 ELISA kitLap-4 ELISA kitLps-Associated Protein 4 ELISA kitRadotermin ELISA kitGDF5 ELISA kitBMP14 ELISA kitCDMP1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
