| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
14.06pg/mL |
| Detection Limit: |
23.44~1500pg/mL |
| Short Description: |
This GDF15 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
GDF15 |
| Gene ID: |
9518 |
| Uniprot ID: |
GDF15_HUMAN |
| Specificity: |
This kit recognizes Human GDF15 in samples. No significant cross-reactivity or interference between Human GDF15 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions. On hepatocytes, inhibits growth hormone signaling. |
| Protein Name | Growth/Differentiation Factor 15Gdf-15Macrophage Inhibitory Cytokine 1Mic-1Nsaid-Activated Gene 1 ProteinNag-1Nsaid-Regulated Gene 1 ProteinNrg-1Placental Tgf-BetaPlacental Bone Morphogenetic ProteinProstate Differentiation Factor |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Growth/Differentiation Factor 15 ELISA kitGdf-15 ELISA kitMacrophage Inhibitory Cytokine 1 ELISA kitMic-1 ELISA kitNsaid-Activated Gene 1 Protein ELISA kitNag-1 ELISA kitNsaid-Regulated Gene 1 Protein ELISA kitNrg-1 ELISA kitPlacental Tgf-Beta ELISA kitPlacental Bone Morphogenetic Protein ELISA kitProstate Differentiation Factor ELISA kitGDF15 ELISA kitMIC1 ELISA kitPDF ELISA kitPLAB ELISA kitPTGFB ELISA kit |
| Specificity | This kit recognizes Human GDF15 in samples. No significant cross-reactivity or interference between Human GDF15 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-103 | 96 |
| EDTA plasma (n=8) | 87-102 | 94 |
| Cell culture media (n=8) | 88-100 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 83.30 | 126.61 | 615.36 | 76.84 | 120.76 | 573.31 |
| Standard deviation | 4.87 | 6.79 | 32.00 | 4.78 | 6.96 | 27.46 |
| CV (%) | 5.85 | 5.36 | 5.20 | 6.22 | 5.76 | 4.79 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
