| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human GATA3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
GATA3 |
| Gene ID: |
2625 |
| Uniprot ID: |
GATA3_HUMAN |
| Specificity: |
This kit recognizes Human GATA3 in samples. No significant cross-reactivity or interference between Human GATA3 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | T-cells and endothelial cells. |
| Function | Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression. Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages. |
| Protein Name | Trans-Acting T-Cell-Specific Transcription Factor Gata-3Gata-Binding Factor 3 |
| Database Links | Reactome: R-HSA-5689880Reactome: R-HSA-6785807Reactome: R-HSA-8939236Reactome: R-HSA-9018519Reactome: R-HSA-983231 |
| Cellular Localisation | Nucleus |
| Alternative CLIA Names | Trans-Acting T-Cell-Specific Transcription Factor Gata-3 CLIA kitGata-Binding Factor 3 CLIA kitGATA3 CLIA kit |
| Specificity | This kit recognizes Human GATA3 in samples. No significant cross-reactivity or interference between Human GATA3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-99 | 92 |
| EDTA plasma(n=8) | 95-108 | 102 |
| Cell culture media(n=8) | 85-99 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 185.12 | 568.83 | 1430.94 | 186.26 | 563.60 | 1531.82 |
| Standard deviation | 16.75 | 61.09 | 136.37 | 22.71 | 65.66 | 163.75 |
| CV (%) | 9.05 | 10.74 | 9.53 | 12.19 | 11.65 | 10.69 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
