| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5ng/mL |
| Detection Limit: |
12.5~800ng/mL |
| Short Description: |
This FTH Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
FTH1 |
| Gene ID: |
2495 |
| Uniprot ID: |
FRIH_HUMAN |
| Specificity: |
This kit recognizes Human FTH in samples. No significant cross-reactivity or interference between Human FTH and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. |
| Protein Name | Ferritin Heavy ChainFerritin H SubunitCell Proliferation-Inducing Gene 15 Protein Cleaved Into - Ferritin Heavy Chain - N-Terminally Processed |
| Database Links | Reactome: R-HSA-3000480Reactome: R-HSA-432722Reactome: R-HSA-6798695Reactome: R-HSA-917937 |
| Cellular Localisation | Cytoplasm |
| Alternative ELISA Names | Ferritin Heavy Chain ELISA kitFerritin H Subunit ELISA kitCell Proliferation-Inducing Gene 15 Protein Cleaved Into - Ferritin Heavy Chain - N-Terminally Processed ELISA kitFTH1 ELISA kitFTH ELISA kitFTHL6 ELISA kitOK ELISA kitSW-cl.84 ELISA kitPIG15 ELISA kit |
| Specificity | This kit recognizes Human FTH in samples. No significant cross-reactivity or interference between Human FTH and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 84-98 | 90 |
| EDTA plasma (n=8) | 89-103 | 95 |
| Cell culture media (n=8) | 88-101 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 38.61 | 98.00 | 378.23 | 40.84 | 105.88 | 387.76 |
| Standard deviation | 2.23 | 5.75 | 12.71 | 2.72 | 6.11 | 15.16 |
| CV (%) | 5.78 | 5.87 | 3.36 | 6.66 | 5.77 | 3.91 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
