| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This human FPR2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
FPR2 |
| Gene ID: |
2358 |
| Uniprot ID: |
FPR2_HUMAN |
| Specificity: |
This kit recognizes Human FPR2 in samples. No significant cross-reactivity or interference between Human FPR2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Detected in lung, bone marrow, neutrophils, spleen and testis. |
| Function | Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophil chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of pro-inflammatory signals such as LTB4 (leukotriene B4). Receptor for the chemokine-like protein FAM19A5, mediating FAM19A5-stimulated macrophage chemotaxis and the inhibitory effect on TNFSF11/RANKL-induced osteoclast differentiation. Acts as a receptor for humanin. |
| Protein Name | N-Formyl Peptide Receptor 2Fmlp-Related Receptor IFmlp-R-IFormyl Peptide Receptor-Like 1Hm63Lipoxin A4 ReceptorLxa4 ReceptorRfp |
| Database Links | Reactome: R-HSA-416476Reactome: R-HSA-418594Reactome: R-HSA-444473Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinAssociates With Amyloid-Beta Protein 42Product Of AppAt The Cell Surface And The Complex Is Then Rapidly InternalizedAlso Internalized In The Presence Of Humanin |
| Alternative CLIA Names | N-Formyl Peptide Receptor 2 CLIA kitFmlp-Related Receptor I CLIA kitFmlp-R-I CLIA kitFormyl Peptide Receptor-Like 1 CLIA kitHm63 CLIA kitLipoxin A4 Receptor CLIA kitLxa4 Receptor CLIA kitRfp CLIA kitFPR2 CLIA kitFPRH1 CLIA kitFPRL1 CLIA kitLXA4R CLIA kit |
| Specificity | This kit recognizes Human FPR2 in samples. No significant cross-reactivity or interference between Human FPR2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 99-116 | 107 |
| EDTA plasma(n=8) | 83-97 | 90 |
| Cell culture media(n=8) | 99-116 | 107 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 47.07 | 114.53 | 429.70 | 47.94 | 104.56 | 397.60 |
| Standard deviation | 4.52 | 11.11 | 44.86 | 4.52 | 7.47 | 28.43 |
| CV (%) | 9.60 | 9.70 | 10.44 | 9.43 | 7.14 | 7.15 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
