| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This FOXP3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
FOXP3 |
| Gene ID: |
50943 |
| Uniprot ID: |
FOXP3_HUMAN |
| Specificity: |
This kit recognizes Human FOXP3 in samples. No significant cross-reactivity or interference between Human FOXP3 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2. Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7. Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1. Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development. Inhibits the transcriptional activator activity of RORA. Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4. |
| Protein Name | Forkhead Box Protein P3Scurfin Cleaved Into - Forkhead Box Protein P3 - C-Terminally Processed - Forkhead Box Protein P3 41 Kda Form |
| Database Links | Reactome: R-HSA-8877330Reactome: R-HSA-8939256 |
| Cellular Localisation | NucleusCytoplasmPredominantly Expressed In The Cytoplasm In Activated Conventional T-Cells Whereas Predominantly Expressed In The Nucleus In Regulatory T-Cells (Treg)The 41 Kda Form Derived By Proteolytic Processing Is Found Exclusively In The Chromatin Fraction Of Activated Treg Cells |
| Alternative ELISA Names | Forkhead Box Protein P3 ELISA kitScurfin Cleaved Into - Forkhead Box Protein P3 - C-Terminally Processed - Forkhead Box Protein P3 41 Kda Form ELISA kitFOXP3 ELISA kitIPEX ELISA kitJM2 ELISA kit |
| Specificity | This kit recognizes Human FOXP3 in samples. No significant cross-reactivity or interference between Human FOXP3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-111 | 101 |
| EDTA plasma (n=8) | 95-109 | 100 |
| Cell culture media (n=8) | 94-111 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.98 | 2.68 | 8.00 | 1.07 | 2.58 | 7.85 |
| Standard deviation | 0.05 | 0.11 | 0.43 | 0.06 | 0.11 | 0.34 |
| CV (%) | 5.10 | 4.10 | 5.38 | 5.61 | 4.26 | 4.33 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
