| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human FOXC2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
FOXC2 |
| Gene ID: |
2303 |
| Uniprot ID: |
FOXC2_HUMAN |
| Specificity: |
This kit recognizes Human FOXC2 in samples. No significant cross-reactivity or interference between Human FOXC2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylation regulates FOXC2 transcriptional activity by promoting its recruitment to chromatin. |
| Function | Transcriptional activator. Might be involved in the formation of special mesenchymal tissues. |
| Protein Name | Forkhead Box Protein C2Forkhead-Related Protein Fkhl14Mesenchyme Fork Head Protein 1Mfh-1 ProteinTranscription Factor Fkh-14 |
| Database Links | Reactome: R-HSA-9761174 |
| Cellular Localisation | Nucleus |
| Alternative CLIA Names | Forkhead Box Protein C2 CLIA kitForkhead-Related Protein Fkhl14 CLIA kitMesenchyme Fork Head Protein 1 CLIA kitMfh-1 Protein CLIA kitTranscription Factor Fkh-14 CLIA kitFOXC2 CLIA kitFKHL14 CLIA kitMFH1 CLIA kit |
| Specificity | This kit recognizes Human FOXC2 in samples. No significant cross-reactivity or interference between Human FOXC2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-104 | 96 |
| EDTA plasma(n=8) | 93-108 | 101 |
| Cell culture media(n=8) | 86-102 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 100.62 | 180.71 | 702.44 | 107.90 | 169.64 | 723.87 |
| Standard deviation | 11.82 | 18.69 | 58.93 | 12.98 | 17.13 | 70.65 |
| CV (%) | 11.75 | 10.34 | 8.39 | 12.03 | 10.10 | 9.76 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
