| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
2.81ng/mL |
| Detection Limit: |
4.69~300ng/mL |
| Short Description: |
This FOLH1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
FOLH1 |
| Gene ID: |
2346 |
| Uniprot ID: |
FOLH1_HUMAN |
| Specificity: |
This kit recognizes Human PSMA in samples. No significant cross-reactivity or interference between Human PSMA and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis. The N-terminus is blocked. |
| Function | Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression. Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. |
| Protein Name | Glutamate Carboxypeptidase 2Cell Growth-Inhibiting Gene 27 ProteinFolate Hydrolase 1Folylpoly-Gamma-Glutamate CarboxypeptidaseFgcpGlutamate Carboxypeptidase IiGcpiiMembrane Glutamate CarboxypeptidaseMgcpN-Acetylated-Alpha-Linked Acidic Dipeptidase INaaladase IProstate-Specific Membrane AntigenPsmPsmaPteroylpoly-Gamma-Glutamate Carboxypeptidase |
| Database Links | Reactome: R-HSA-8963693 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinIsoform Psma': Cytoplasm |
| Alternative ELISA Names | Glutamate Carboxypeptidase 2 ELISA kitCell Growth-Inhibiting Gene 27 Protein ELISA kitFolate Hydrolase 1 ELISA kitFolylpoly-Gamma-Glutamate Carboxypeptidase ELISA kitFgcp ELISA kitGlutamate Carboxypeptidase Ii ELISA kitGcpii ELISA kitMembrane Glutamate Carboxypeptidase ELISA kitMgcp ELISA kitN-Acetylated-Alpha-Linked Acidic Dipeptidase I ELISA kitNaaladase I ELISA kitProstate-Specific Membrane Antigen ELISA kitPsm ELISA kitPsma ELISA kitPteroylpoly-Gamma-Glutamate Carboxypeptidase ELISA kitFOLH1 ELISA kitFOLH ELISA kitNAALAD1 ELISA kitPSM ELISA kitPSMA ELISA kitGIG27 ELISA kit |
| Specificity | This kit recognizes Human PSMA in samples. No significant cross-reactivity or interference between Human PSMA and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-102 | 95 |
| EDTA plasma (n=8) | 84-100 | 91 |
| Cell culture media (n=8) | 88-100 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 15.17 | 30.80 | 142.79 | 13.66 | 27.97 | 143.14 |
| Standard deviation | 0.91 | 1.55 | 7.21 | 0.93 | 1.50 | 4.65 |
| CV (%) | 6.00 | 5.03 | 5.05 | 6.81 | 5.36 | 3.25 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
