• Human FN1 (Fibronectin) CLIA Kit (STJC0000141)

Human FN1 (Fibronectin) CLIA Kit (STJC0000141)

SKU:
STJC0000141

$321.98 - $644.63
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This human FN1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: FN1
Gene ID: 2335
Uniprot ID: FINC_HUMAN
Specificity: This kit recognizes Human FN in samples. No significant cross-reactivity or interference between Human FN and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in the inner limiting membrane and around blood vessels in the retina (at protein level). Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
Post Translational Modifications Sulfated. It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylated by FAM20C in the extracellular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation. Serotonylated on Gln residues by TGM2 in response to hypoxia.
Function Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation. Anastellin: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.
Protein Name Fibronectin
Fn
Cold-Insoluble Globulin
Cig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1474228
Reactome: R-HSA-1474244
Reactome: R-HSA-1566977
Reactome: R-HSA-202733
Reactome: R-HSA-2129379
Reactome: R-HSA-216083
Reactome: R-HSA-3000170
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-354192
Reactome: R-HSA-354194
Reactome: R-HSA-372708
Reactome: R-HSA-381426
Reactome: R-HSA-5674135
Reactome: R-HSA-6785807
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-8874081
Reactome: R-HSA-8957275
Reactome: R-HSA-9634597
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9700645
Reactome: R-HSA-9725370
Reactome: R-HSA-9725371
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative CLIA Names Fibronectin CLIA kit
Fn CLIA kit
Cold-Insoluble Globulin CLIA kit
Cig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3 CLIA kit
FN1 CLIA kit
FN CLIA kit
Specificity This kit recognizes Human FN in samples. No significant cross-reactivity or interference between Human FN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-104 95
EDTA plasma(n=8) 96-112 104
Cell culture media(n=8) 95-109 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.94 2.31 6.82 0.94 2.20 7.17
Standard deviation 0.10 0.22 0.55 0.08 0.25 0.54
CV (%) 10.64 9.52 8.06 8.51 11.36 7.53
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance