| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This human FN1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
FN1 |
| Gene ID: |
2335 |
| Uniprot ID: |
FINC_HUMAN |
| Specificity: |
This kit recognizes Human FN in samples. No significant cross-reactivity or interference between Human FN and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in the inner limiting membrane and around blood vessels in the retina (at protein level). Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. |
| Post Translational Modifications | Sulfated. It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylated by FAM20C in the extracellular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation. Serotonylated on Gln residues by TGM2 in response to hypoxia. |
| Function | Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation. Anastellin: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling. |
| Protein Name | FibronectinFnCold-Insoluble GlobulinCig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1474228Reactome: R-HSA-1474244Reactome: R-HSA-1566977Reactome: R-HSA-202733Reactome: R-HSA-2129379Reactome: R-HSA-216083Reactome: R-HSA-3000170Reactome: R-HSA-3000171Reactome: R-HSA-3000178Reactome: R-HSA-354192Reactome: R-HSA-354194Reactome: R-HSA-372708Reactome: R-HSA-381426Reactome: R-HSA-5674135Reactome: R-HSA-6785807Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-8874081Reactome: R-HSA-8957275Reactome: R-HSA-9634597Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9700645Reactome: R-HSA-9725370Reactome: R-HSA-9725371 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative CLIA Names | Fibronectin CLIA kitFn CLIA kitCold-Insoluble Globulin CLIA kitCig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3 CLIA kitFN1 CLIA kitFN CLIA kit |
| Specificity | This kit recognizes Human FN in samples. No significant cross-reactivity or interference between Human FN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-104 | 95 |
| EDTA plasma(n=8) | 96-112 | 104 |
| Cell culture media(n=8) | 95-109 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.94 | 2.31 | 6.82 | 0.94 | 2.20 | 7.17 |
| Standard deviation | 0.10 | 0.22 | 0.55 | 0.08 | 0.25 | 0.54 |
| CV (%) | 10.64 | 9.52 | 8.06 | 8.51 | 11.36 | 7.53 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
