| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description: | This FGF2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | FGF2 |
| Gene ID: | 2247 |
| Uniprot ID: | FGF2_HUMAN |
| Specificity: | This kit recognizes Human bFGF/FGF2 in samples. No significant cross-reactivity or interference between Human bFGF/FGF2 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. |
| Function | Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation. |
| Protein Name | Fibroblast Growth Factor 2Fgf-2Basic Fibroblast Growth FactorBfgfHeparin-Binding Growth Factor 2Hbgf-2 |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-1257604Reactome: R-HSA-1839122Reactome: R-HSA-1839130Reactome: R-HSA-190322Reactome: R-HSA-190370Reactome: R-HSA-190372Reactome: R-HSA-190373Reactome: R-HSA-190375Reactome: R-HSA-190377Reactome: R-HSA-2033519Reactome: R-HSA-2219530Reactome: R-HSA-2892247Reactome: R-HSA-3000170Reactome: R-HSA-3000171Reactome: R-HSA-5654219Reactome: R-HSA-5654221Reactome: R-HSA-5654227Reactome: R-HSA-5654228Reactome: R-HSA-5654687Reactome: R-HSA-5654688Reactome: R-HSA-5654689Reactome: R-HSA-5654693Reactome: R-HSA-5654695Reactome: R-HSA-5654699Reactome: R-HSA-5654700Reactome: R-HSA-5654704Reactome: R-HSA-5654706Reactome: R-HSA-5654710Reactome: R-HSA-5654712Reactome: R-HSA-5654719Reactome: R-HSA-5654720Reactome: R-HSA-5654726Reactome: R-HSA-5654727Reactome: R-HSA-5654732Reactome: R-HSA-5654733Reactome: R-HSA-5655253Reactome: R-HSA-5655302Reactome: R-HSA-5655332Reactome: R-HSA-5658623Reactome: R-HSA-5673001Reactome: R-HSA-6785807Reactome: R-HSA-6811558Reactome: R-HSA-8851708Reactome: R-HSA-9761174 |
| Cellular Localisation | SecretedNucleusExported From Cells By An Endoplasmic Reticulum (Er)/Golgi-Independent MechanismUnconventional Secretion Of Fgf2 Occurs By Direct Translocation Across The Plasma MembraneBinding Of Exogenous Fgf2 To Fgfr Facilitates Endocytosis Followed By Translocation Of Fgf2 Across Endosomal Membrane Into The CytosolNuclear Import From The Cytosol Requires The Classical Nuclear Import MachineryInvolving Proteins Kpna1 And Kpnb1As Well As Cep57 |
| Alternative ELISA Names | Fibroblast Growth Factor 2 ELISA kitFgf-2 ELISA kitBasic Fibroblast Growth Factor ELISA kitBfgf ELISA kitHeparin-Binding Growth Factor 2 ELISA kitHbgf-2 ELISA kitFGF2 ELISA kitFGFB ELISA kit |
| Specificity | This kit recognizes Human bFGF/FGF2 in samples. No significant cross-reactivity or interference between Human bFGF/FGF2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 95-105 | 100 |
| EDTA plasma (n=8) | 87-99 | 94 |
| Cell culture media (n=8) | 87-103 | 95 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 83.31 | 221.04 | 991.47 | 81.61 | 245.82 | 870.55 |
| Standard deviation | 4.48 | 10.68 | 48.09 | 4.63 | 11.14 | 36.39 |
| CV (%) | 5.38 | 4.83 | 4.85 | 5.67 | 4.53 | 4.18 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
