| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.38ng/mL |
| Detection Limit: |
0.63~40ng/mL |
| Short Description: |
This human FBLN5 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
FBLN5 |
| Gene ID: |
10516 |
| Uniprot ID: |
FBLN5_HUMAN |
| Specificity: |
This kit recognizes Human FBLN5 in samples. No significant cross-reactivity or interference between Human FBLN5 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in skin fibroblasts (at protein level). Expressed predominantly in heart, ovary, and colon but also in kidney, pancreas, testis, lung and placenta. Not detectable in brain, liver, thymus, prostate, or peripheral blood leukocytes. |
| Post Translational Modifications | N-glycosylated. |
| Function | Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN. Stabilizes and organizes elastic fibers in the skin, lung and vasculature. Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Vascular ligand for integrin receptors which may play a role in vascular development and remodeling. May act as an adapter that mediates the interaction between FBN1 and ELN. |
| Protein Name | Fibulin-5Fibl-5Developmental Arteries And Neural Crest Egf-Like ProteinDanceUrine P50 ProteinUp50 |
| Database Links | Reactome: R-HSA-1566948Reactome: R-HSA-2129379 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixCo-Localizes With Eln In Elastic Fibers |
| Alternative CLIA Names | Fibulin-5 CLIA kitFibl-5 CLIA kitDevelopmental Arteries And Neural Crest Egf-Like Protein CLIA kitDance CLIA kitUrine P50 Protein CLIA kitUp50 CLIA kitFBLN5 CLIA kitDANCE CLIA kitUNQ184 CLIA kitPRO210 CLIA kit |
| Specificity | This kit recognizes Human FBLN5 in samples. No significant cross-reactivity or interference between Human FBLN5 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-103 | 98 |
| EDTA plasma(n=8) | 90-105 | 96 |
| Cell culture media(n=8) | 89-104 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.01 | 4.64 | 16.36 | 2.03 | 5.09 | 17.42 |
| Standard deviation | 0.19 | 0.41 | 1.66 | 0.22 | 0.52 | 1.56 |
| CV (%) | 9.45 | 8.84 | 10.15 | 10.84 | 10.22 | 8.96 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
