| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.75ng/mL |
| Detection Limit: |
1.25~80ng/mL |
| Short Description: |
This F9 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
F9 |
| Gene ID: |
2158 |
| Uniprot ID: |
FA9_HUMAN |
| Specificity: |
This kit recognizes Human F9 in samples. No significant cross-reactivity or interference between Human F9 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Activated by factor XIa, which excises the activation peptide. The propeptide can also be removed by snake venom protease. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. |
| Function | Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. |
| Protein Name | Coagulation Factor IxChristmas FactorPlasma Thromboplastin ComponentPtc Cleaved Into - Coagulation Factor Ixa Light Chain - Coagulation Factor Ixa Heavy Chain |
| Database Links | Reactome: R-HSA-140834Reactome: R-HSA-140837Reactome: R-HSA-159740Reactome: R-HSA-159763Reactome: R-HSA-159782Reactome: R-HSA-9672383Reactome: R-HSA-9672396Reactome: R-HSA-9673202Reactome: R-HSA-9673218Reactome: R-HSA-9673221Reactome: R-HSA-9673240 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Coagulation Factor Ix ELISA kitChristmas Factor ELISA kitPlasma Thromboplastin Component ELISA kitPtc Cleaved Into - Coagulation Factor Ixa Light Chain - Coagulation Factor Ixa Heavy Chain ELISA kitF9 ELISA kit |
| Specificity | This kit recognizes Human F9 in samples. No significant cross-reactivity or interference between Human F9 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-107 | 100 |
| EDTA plasma (n=8) | 93-105 | 99 |
| Cell culture media (n=8) | 84-97 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 4.40 | 12.40 | 31.00 | 4.30 | 11.30 | 28.00 |
| Standard deviation | 0.20 | 0.70 | 1.50 | 0.30 | 0.70 | 1.40 |
| CV (%) | 4.55 | 5.65 | 4.84 | 6.98 | 6.19 | 5.00 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
