| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69pg/mL |
| Detection Limit: |
7.81~500pg/mL |
| Short Description: |
This human F3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
F3 |
| Gene ID: |
2152 |
| Uniprot ID: |
TF_HUMAN |
| Specificity: |
This kit recognizes Human TF in samples. No significant cross-reactivity or interference between Human TF and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Lung, placenta and pancreas. |
| Function | Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The TF:VIIa complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. |
| Protein Name | Tissue FactorTfCoagulation Factor IiiThromboplastinCd Antigen Cd142 |
| Database Links | Reactome: R-HSA-140834Reactome: R-HSA-9031628 |
| Cellular Localisation | Isoform 1: MembraneSingle-Pass Type I Membrane ProteinIsoform 2: Secreted |
| Alternative CLIA Names | Tissue Factor CLIA kitTf CLIA kitCoagulation Factor Iii CLIA kitThromboplastin CLIA kitCd Antigen Cd142 CLIA kitF3 CLIA kit |
| Specificity | This kit recognizes Human TF in samples. No significant cross-reactivity or interference between Human TF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 102-115 | 108 |
| EDTA plasma(n=8) | 92-109 | 100 |
| Cell culture media(n=8) | 95-110 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 23.81 | 52.44 | 218.76 | 23.18 | 52.30 | 231.75 |
| Standard deviation | 2.58 | 5.68 | 19.49 | 2.71 | 5.47 | 25.40 |
| CV (%) | 10.84 | 10.83 | 8.91 | 11.69 | 10.46 | 10.96 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
