| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This human F13A1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
F13A1 |
| Gene ID: |
2162 |
| Uniprot ID: |
F13A_HUMAN |
| Specificity: |
This kit recognizes Human F13A1 in samples. No significant cross-reactivity or interference between Human F13A1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The activation peptide is released by thrombin. |
| Function | Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. |
| Protein Name | Coagulation Factor Xiii A ChainCoagulation Factor XiiiaProtein-Glutamine Gamma-Glutamyltransferase A ChainTransglutaminase A Chain |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-140875Reactome: R-HSA-6785807 |
| Cellular Localisation | CytoplasmSecretedSecreted Into The Blood PlasmaCytoplasmic In Most TissuesBut Also Secreted In The Blood Plasma |
| Alternative CLIA Names | Coagulation Factor Xiii A Chain CLIA kitCoagulation Factor Xiiia CLIA kitProtein-Glutamine Gamma-Glutamyltransferase A Chain CLIA kitTransglutaminase A Chain CLIA kitF13A1 CLIA kitF13A CLIA kit |
| Specificity | This kit recognizes Human F13A1 in samples. No significant cross-reactivity or interference between Human F13A1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-99 | 92 |
| EDTA plasma(n=8) | 91-103 | 98 |
| Cell culture media(n=8) | 85-97 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 37.66 | 72.96 | 305.26 | 40.70 | 73.29 | 277.75 |
| Standard deviation | 4.56 | 7.30 | 34.40 | 3.51 | 6.63 | 23.64 |
| CV (%) | 12.11 | 10.01 | 11.27 | 8.62 | 9.05 | 8.51 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
