• Human EZH2 (Enhancer Of Zeste Homolog 2) Sandwich ELISA Kit Ready-To-Use (STJE0015103)

Human EZH2 (Enhancer Of Zeste Homolog 2) Sandwich ELISA Kit Ready-To-Use (STJE0015103)

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STJE0015103

$650.03 - $890.33
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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.058ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This EZH2 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: EZH2
Gene ID: 2146
Uniprot ID: EZH2_HUMAN
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity In the ovary, expressed in primordial follicles and oocytes and also in external follicle cells (at protein level). Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
Post Translational Modifications Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. Phosphorylation at Thr-345 by CDK1 and CDK2 promotes maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing. Sumoylated. Glycosylated: O-GlcNAcylation at Ser-75 by OGT increases stability of EZH2 and facilitates the formation of H3K27me3 by the PRC2/EED-EZH2 complex.
Function Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-BMAL1 heterodimer.involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
Protein Name Histone-Lysine N-Methyltransferase Ezh2
Enx-1
Enhancer Of Zeste Homolog 2
Lysine N-Methyltransferase 6
Database Links Reactome: R-HSA-212300
Reactome: R-HSA-2559580
Reactome: R-HSA-3214841
Reactome: R-HSA-5617472
Reactome: R-HSA-8943724
Reactome: R-HSA-8953750
Reactome: R-HSA-9609690
Reactome: R-HSA-9710421
Cellular Localisation Nucleus
Localizes To The Inactive X Chromosome In Trophoblast Stem Cells
Alternative ELISA Names Histone-Lysine N-Methyltransferase Ezh2 ELISA kit
Enx-1 ELISA kit
Enhancer Of Zeste Homolog 2 ELISA kit
Lysine N-Methyltransferase 6 ELISA kit
EZH2 ELISA kit
KMT6 ELISA kit
output

Information sourced from Uniprot.org

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