Applications: | ELISA |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Sensitivity: | 34.1 pg/mL |
Detection Limit: | 78-5000 pg/ml |
Short Description: | This EPOR Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Conjugation: | Biotin |
Storage Instruction: | Unopened kits can be stored at 2-8°C for 1 year, and opened products must be used within 1 month. |
Assay Time: | 4 hours |
Detection: | Colorimetric |
Gene Symbol: | EPOR |
Gene ID: | 2057 |
Uniprot ID: | EPOR_HUMAN |
Sample Type: | serum, plasma and cell culture supernates |
Tissue Specificity | Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells. |
Post Translational Modifications | On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site. Ubiquitination at Lys-281 mediates receptor internalization, whereas ubiquitination at Lys-453 promotes trafficking of activated receptors to the lysosomes for degradation. Ubiquitinated by NOSIP.appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells. |
Function | Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase. Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling. |
Protein Name | Erythropoietin Receptor Epo-R |
Database Links | Reactome: R-HSA-9006335 Reactome: R-HSA-9027276 Reactome: R-HSA-9027277 Reactome: R-HSA-9027283 Reactome: R-HSA-9027284 |
Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Isoform Epor-S: Secreted Secreted And Located To The Cell Surface |
Alternative ELISA Names | Erythropoietin Receptor ELISA kit Epo-R ELISA kit EPOR ELISA kit |
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Information sourced from Uniprot.org