| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19IU/mL |
| Detection Limit: |
0.31~20IU/mL |
| Short Description: |
This human EPCAM kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
EPCAM |
| Gene ID: |
4072 |
| Uniprot ID: |
EPCAM_HUMAN |
| Specificity: |
This kit recognizes Human Ep-CAM/CD326 in samples. No significant cross-reactivity or interference between Human Ep-CAM/CD326 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. |
| Post Translational Modifications | Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. |
| Function | May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. |
| Protein Name | Epithelial Cell Adhesion MoleculeEp-CamAdenocarcinoma-Associated AntigenCell Surface Glycoprotein Trop-1Epithelial Cell Surface AntigenEpithelial GlycoproteinEgpEpithelial Glycoprotein 314Egp314Hegp314Ks 1/4 AntigenKsaMajor Gastrointestinal Tumor-Associated Protein Ga733-2Tumor-Associated Calcium Signal Transducer 1Cd Antigen Cd326 |
| Database Links | Reactome: R-HSA-202733 |
| Cellular Localisation | Lateral Cell MembraneSingle-Pass Type I Membrane ProteinCell JunctionTight JunctionColocalizes With Cldn7 At The Lateral Cell Membrane And Tight Junction |
| Alternative CLIA Names | Epithelial Cell Adhesion Molecule CLIA kitEp-Cam CLIA kitAdenocarcinoma-Associated Antigen CLIA kitCell Surface Glycoprotein Trop-1 CLIA kitEpithelial Cell Surface Antigen CLIA kitEpithelial Glycoprotein CLIA kitEgp CLIA kitEpithelial Glycoprotein 314 CLIA kitEgp314 CLIA kitHegp314 CLIA kitKs 1/4 Antigen CLIA kitKsa CLIA kitMajor Gastrointestinal Tumor-Associated Protein Ga733-2 CLIA kitTumor-Associated Calcium Signal Transducer 1 CLIA kitCd Antigen Cd326 CLIA kitEPCAM CLIA kitGA733-2 CLIA kitM1S2 CLIA kitM4S1 CLIA kitMIC18 CLIA kitTACSTD1 CLIA kitTROP1 CLIA kit |
| Specificity | This kit recognizes Human Ep-CAM/CD326 in samples. No significant cross-reactivity or interference between Human Ep-CAM/CD326 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-107 | 100 |
| EDTA plasma(n=8) | 87-97 | 92 |
| Cell culture media(n=8) | 96-114 | 104 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (IU/mL) | 0.91 | 1.91 | 7.30 | 0.86 | 1.75 | 7.13 |
| Standard deviation | 0.09 | 0.22 | 0.75 | 0.07 | 0.14 | 0.49 |
| CV (%) | 9.89 | 11.52 | 10.27 | 8.14 | 8.00 | 6.87 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
