| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This EPAS1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
EPAS1 |
| Gene ID: |
2034 |
| Uniprot ID: |
EPAS1_HUMAN |
| Specificity: |
This kit recognizes Human HIF-2 Alpha in samples. No significant cross-reactivity or interference between Human HIF-2 Alpha and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. Phosphorylated on multiple sites in the CTAD. The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. |
| Function | Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT.heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD. |
| Protein Name | Endothelial Pas Domain-Containing Protein 1Epas-1Basic-Helix-Loop-Helix-Pas Protein Mop2Class E Basic Helix-Loop-Helix Protein 73Bhlhe73Hif-1-Alpha-Like FactorHlfHypoxia-Inducible Factor 2-AlphaHif-2-AlphaHif2-AlphaMember Of Pas Protein 2Pas Domain-Containing Protein 2 |
| Database Links | Reactome: R-HSA-1234158Reactome: R-HSA-1234174Reactome: R-HSA-1234176Reactome: R-HSA-452723Reactome: R-HSA-8849473Reactome: R-HSA-8951664Reactome: R-HSA-9664873 |
| Cellular Localisation | NucleusNucleus SpeckleColocalizes With Hif3a In The Nucleus And Speckles |
| Alternative ELISA Names | Endothelial Pas Domain-Containing Protein 1 ELISA kitEpas-1 ELISA kitBasic-Helix-Loop-Helix-Pas Protein Mop2 ELISA kitClass E Basic Helix-Loop-Helix Protein 73 ELISA kitBhlhe73 ELISA kitHif-1-Alpha-Like Factor ELISA kitHlf ELISA kitHypoxia-Inducible Factor 2-Alpha ELISA kitHif-2-Alpha ELISA kitHif2-Alpha ELISA kitMember Of Pas Protein 2 ELISA kitPas Domain-Containing Protein 2 ELISA kitEPAS1 ELISA kitBHLHE73 ELISA kitHIF2A ELISA kitMOP2 ELISA kitPASD2 ELISA kit |
| Specificity | This kit recognizes Human HIF-2α in samples. No significant cross-reactivity or interference between Human HIF-2α and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-110 | 101 |
| EDTA plasma (n=8) | 94-112 | 102 |
| Cell culture media (n=8) | 88-102 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.48 | 1.59 | 3.82 | 0.45 | 1.72 | 3.58 |
| Standard deviation | 0.02 | 0.08 | 0.20 | 0.02 | 0.10 | 0.16 |
| CV (%) | 4.17 | 5.03 | 5.24 | 4.44 | 5.81 | 4.47 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
