| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.118ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This ELAVL1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
ELAVL1 |
| Gene ID: |
1994 |
| Uniprot ID: |
ELAV1_HUMAN |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Ubiquitous. Detected in brain, liver, thymus and muscle. |
| Post Translational Modifications | Phosphorylated by MAPKAPK2. Phosphorylated by PRKCD. Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. |
| Function | RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation. Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUUAGUUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA. Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR. |
| Protein Name | Elav-Like Protein 1Hu-Antigen RHur |
| Database Links | Reactome: R-HSA-450520 |
| Cellular Localisation | CytoplasmNucleusStress GranuleP-BodyTranslocates Into The Cytoplasm Following Phosphorylation By Mapkapk2LikewisePhosphorylation By Prkcd Promotes Translocation From The Nucleus Into The CytoplasmWhere It Is Associated With Free And Cytoskeleton-Bound PolysomesLocalizes To The Stress Granules In The Presence Of Plekhn1 |
| Alternative ELISA Names | Elav-Like Protein 1 ELISA kitHu-Antigen R ELISA kitHur ELISA kitELAVL1 ELISA kitHUR ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
