| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.38ng/mL |
| Detection Limit: |
0.63~40ng/mL |
| Short Description: |
This human EFEMP2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
EFEMP2 |
| Gene ID: |
30008 |
| Uniprot ID: |
FBLN4_HUMAN |
| Specificity: |
This kit recognizes Human FBLN4 in samples. No significant cross-reactivity or interference between Human FBLN4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N-glycosylated.contains mostly complex-type glycans. Not O-glycosylated. Cleaved by ELANE.produces a 50-55 kDa fragment. Cleaved by MMP2 and MMP9.produces several fragments. |
| Function | Plays a crucial role in elastic fiber formation in tissue, and in the formation of ultrastructural connections between elastic laminae and smooth muscle cells in the aorta, therefore participates in terminal differentiation and maturation of smooth muscle cell (SMC) and in the mechanical properties and wall integrity maintenance of the aorta. In addition, is involved in the control of collagen fibril assembly in tissue throught proteolytic activation of LOX leading to cross- linking of collagen and elastin. Also promotes ELN coacervation and participates in the deposition of ELN coacervates on to microfibrils but also regulates ELN cross- linking through LOX interaction. Moreover adheres to the cells through heparin binding in a calcium-dependent manner and regulates vascularlar smooth muscle cells proliferation through angiotensin signaling. |
| Protein Name | Egf-Containing Fibulin-Like Extracellular Matrix Protein 2Fibulin-4Fibl-4Protein Uph1 |
| Database Links | Reactome: R-HSA-2129379 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneLocalizes On The Microfibrils Surrounding Eln Cores |
| Alternative CLIA Names | Egf-Containing Fibulin-Like Extracellular Matrix Protein 2 CLIA kitFibulin-4 CLIA kitFibl-4 CLIA kitProtein Uph1 CLIA kitEFEMP2 CLIA kitFBLN4 CLIA kitUNQ200 CLIA kitPRO226 CLIA kit |
| Specificity | This kit recognizes Human FBLN4 in samples. No significant cross-reactivity or interference between Human FBLN4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-104 | 98 |
| EDTA plasma(n=8) | 88-100 | 94 |
| Cell culture media(n=8) | 90-103 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.85 | 5.34 | 16.51 | 2.03 | 5.72 | 16.91 |
| Standard deviation | 0.19 | 0.62 | 1.45 | 0.24 | 0.47 | 1.94 |
| CV (%) | 10.27 | 11.61 | 8.78 | 11.82 | 8.22 | 11.47 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
