| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.75pg/mL |
| Detection Limit: |
1.25~80pg/mL |
| Short Description: |
This EDN1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
EDN1 |
| Gene ID: |
1906 |
| Uniprot ID: |
EDN1_HUMAN |
| Specificity: |
This kit recognizes Human ET-1 in samples. No significant cross-reactivity or interference between Human ET-1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells. Also binds the DEAR/FBXW7-AS1 receptor. |
| Protein Name | Endothelin-1Preproendothelin-1Ppet1 Cleaved Into - Endothelin-1Et-1 - Big Endothelin-1 |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-416476 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Endothelin-1 ELISA kitPreproendothelin-1 ELISA kitPpet1 Cleaved Into - Endothelin-1 ELISA kitEt-1 - Big Endothelin-1 ELISA kitEDN1 ELISA kit |
| Specificity | This kit recognizes Human ET-1 in samples. No significant cross-reactivity or interference between Human ET-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-103 | 97 |
| EDTA plasma (n=8) | 89-104 | 96 |
| Cell culture media (n=8) | 86-100 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 4.00 | 7.80 | 33.60 | 4.00 | 7.70 | 36.30 |
| Standard deviation | 0.30 | 0.40 | 1.40 | 0.20 | 0.30 | 1.40 |
| CV (%) | 7.50 | 5.13 | 4.17 | 5.00 | 3.90 | 3.86 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
