| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This human ECM1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
ECM1 |
| Gene ID: |
1893 |
| Uniprot ID: |
ECM1_HUMAN |
| Specificity: |
This kit recognizes Human ECM1 in samples. No significant cross-reactivity or interference between Human ECM1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin.wide expression is observed throughout the dermis with minimal expression in the epidermis. |
| Function | Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. |
| Protein Name | Extracellular Matrix Protein 1Secretory Component P85 |
| Database Links | Reactome: R-HSA-114608 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative CLIA Names | Extracellular Matrix Protein 1 CLIA kitSecretory Component P85 CLIA kitECM1 CLIA kit |
| Specificity | This kit recognizes Human ECM1 in samples. No significant cross-reactivity or interference between Human ECM1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-101 | 94 |
| EDTA plasma(n=8) | 96-110 | 103 |
| Cell culture media(n=8) | 89-105 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 41.02 | 79.36 | 381.87 | 44.08 | 80.36 | 385.09 |
| Standard deviation | 4.88 | 7.56 | 33.64 | 4.82 | 7.88 | 33.43 |
| CV (%) | 11.90 | 9.53 | 8.81 | 10.93 | 9.81 | 8.68 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
