| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.052ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This DHH Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
DHH |
| Gene ID: |
50846 |
| Uniprot ID: |
DHH_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Desert hedgehog protein: Partially autoproteolyzed. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (DhhN). Desert hedgehog protein N-product: N-palmitoylation by HHAT of DhhN is required for desert hedgehog protein N-product multimerization and full activity. |
| Function | Desert hedgehog protein: The C-terminal part of the desert hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (N-product and C-product) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product. Both activities occur in the reticulum endoplasmic. Functions in cell-cell mediated juxtacrine signaling. Promotes endothelium integrity. Binds to PTCH1 receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes in endothelial cells. In Schwann cells, controls the development of the peripheral nerve sheath and the transition of mesenchymal cells to form the epithelium-like structure of the perineurial tube. Desert hedgehog protein N-product: The dually lipidated desert hedgehog protein N-product is essential for a variety of patterning events during development. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Required for normal testis development and spermatogenesis, namely for the formation of adult-type Leydig cells and normal development of peritubular cells and seminiferous tubules. Activates primary cilia signaling on neighboring valve interstitial cells through a paracrine mechanism. May induce motor neurons in lateral neural tube and may have a polarizing activity. Prevents the desert hedgehog protein precursor binding to PTCH1. |
| Protein Name | Desert Hedgehog ProteinDhhHhg-3 Cleaved Into - Desert Hedgehog Protein N-ProductDhh-N |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-5358346Reactome: R-HSA-5362798Reactome: R-HSA-5632681Reactome: R-HSA-5632684Reactome: R-HSA-5635838Reactome: R-HSA-5658034Reactome: R-HSA-9690406 |
| Cellular Localisation | Desert Hedgehog Protein N-Product: Cell MembraneLipid-AnchorDesert Hedgehog Protein: Endoplasmic Reticulum MembraneGolgi Apparatus MembraneSecretedCell MembraneCo-Localizes With Hhat In The Er And Golgi Membrane |
| Alternative ELISA Names | Desert Hedgehog Protein ELISA kitDhh ELISA kitHhg-3 Cleaved Into - Desert Hedgehog Protein N-Product ELISA kitDhh-N ELISA kitDHH ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
