| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38ng/mL |
| Detection Limit: |
15.63~1000ng/mL |
| Short Description: |
This DHEA-S Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
2.5h |
| Detection: |
Colormetric |
| Specificity: |
This kit recognizes Human DHEA-S in samples. No significant cross-reactivity or interference between Human DHEA-S and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Background | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human DHEA-S. During the reaction, Human DHEA-S in the sample or standard competes with a fixed amount of Human DHEA-S on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human DHEA-S. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human DHEA-S in the samples is then determined by comparing the OD of the samples to the standard curve. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
